DITING WITHIN THE FRAMEWORK OF USING PRIME EDITING APPLICATIONS.

Anastasia Korolova
№ 7 Gymnasium School in Khmelnytskyi


Keywords: functional genomics; genome-editing technology; multiplexed assay of variant effect; precision medicine; prime editing; saturation mutagenesis.

Abstract

The key features of the mechanism are valid today.

Single-stranded nicking: Unlike classical CRISPR-Cas9, which causes double-strand breaks, the base editor uses an engineered Cas9 nickase to open only one strand at the target locus.

Single-strand nicking prevents double-strand breaks (DSBs). By using an engineered Cas9 nickase (such as a D10A mutant), the base editor opens only one strand at the target locus. This targeted single-strand break (SSB) or “nick” avoids the genotoxicity and unpredictable insertion/deletion (indel) mutations tied to classic DSB repair.

https://doi.org/10.67626/2rT6m9CjL8

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